CULTIVATION OF THE ORCHID ANОECTOCHILUS ROXBURGHII (WALL.) EX VITRO 

Bol'shakova Ekaterina Viktorovna, Postgraduate student, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail: Fateeva.ek.v@yandex.ru
Emel'yanova Irina Sergeevna, Postgraduate student, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail: emelyanovairina08@rambler.ru
Lukatkin Aleksandr Stepanovich, Doctor of biological sciences, professor, head of sub-department of botany, physiology and ecology of plants, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail: aslukatkin@yandex.ru 

57.042 

DOI

10.21685/2307-9150-2019-3-2 

Background. One of the most difficult and time-consuming stages in clonal propagation of regenerated plants is their transfer into ex vitro conditions and adaptation to exogenous factors. The aim of the work was to determine the most appropriate concentration of indoleacetic acid (IAA) and the exposure duration to survival, morphogenesis and growth of the orchid Anoectochilus roxburghii (Walls.) ex vitro.
Materials and methods. Test-tube plants Anoectochilus roxburghii (Wall.) was planted in a universal soil for orchids with pretreatment with IAA solution at a concentration from 0 to 1,0 mg/L for 15 and 30 minutes, followed by determination of survival, growth and organogenesis. Data analysis was carried out using Microsoft Excel and Statistica applications.
Results. It is revealed that exposure of orchids in IAA solutions has the best survival and growth of plants in conditions ex vitro than transplantation of regenerated plants without IAA pretreatment.
Conclusions. This research showed that a 15-minute exposure of clonally propagated plants Anoectochilus roxburghii in 0,5 mg/L IAA solution revealed the best plants response to transfer in non-sterile conditions. 

Key words

Anoectochilus roxburghii (Wall.), in vitro culture, ex vitro, indoleacetic acid, survival, morphogenesis, growth 

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